Sensitive detection of Pneumocystis jirovecii DNA in gastric wash using nested polymerase chain reaction.
Southeast Asian J Trop Med Public Health
;
2007 Sep; 38(5): 892-6
Article
in English
| IMSEAR
| ID: sea-35838
ABSTRACT
One hundred and five samples of gastric washes were obtained from 52 pediatric patients. Eleven of the 105 samples (10%) gave positive results using immunofluorescence antibody test (IFA) for Pneumocystis jirovecii. Single-step polymerase chain reaction (PCR) produced 13% (14 samples), whereas detection by nested PCR was increased to 65 samples (62%). Moderate agreement (kappa = 0.5) was found between test results of IFA and single-step PCR, but no agreement was found between the results of IFA and nested PCR (kappa = 0.1).
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Pneumonia, Pneumocystis
/
Humans
/
Child
/
Polymerase Chain Reaction
/
Fluorescent Antibody Technique
/
Sensitivity and Specificity
/
Pneumocystis carinii
/
Gastric Mucosa
Type of study:
Diagnostic study
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2007
Type:
Article
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