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Sensitive detection of Pneumocystis jirovecii DNA in gastric wash using nested polymerase chain reaction.
Southeast Asian J Trop Med Public Health ; 2007 Sep; 38(5): 892-6
Article in English | IMSEAR | ID: sea-35838
ABSTRACT
One hundred and five samples of gastric washes were obtained from 52 pediatric patients. Eleven of the 105 samples (10%) gave positive results using immunofluorescence antibody test (IFA) for Pneumocystis jirovecii. Single-step polymerase chain reaction (PCR) produced 13% (14 samples), whereas detection by nested PCR was increased to 65 samples (62%). Moderate agreement (kappa = 0.5) was found between test results of IFA and single-step PCR, but no agreement was found between the results of IFA and nested PCR (kappa = 0.1).
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Pneumonia, Pneumocystis / Humans / Child / Polymerase Chain Reaction / Fluorescent Antibody Technique / Sensitivity and Specificity / Pneumocystis carinii / Gastric Mucosa Type of study: Diagnostic study Language: English Journal: Southeast Asian J Trop Med Public Health Year: 2007 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Pneumonia, Pneumocystis / Humans / Child / Polymerase Chain Reaction / Fluorescent Antibody Technique / Sensitivity and Specificity / Pneumocystis carinii / Gastric Mucosa Type of study: Diagnostic study Language: English Journal: Southeast Asian J Trop Med Public Health Year: 2007 Type: Article