Antibiotic resistance, plasmid profile and RAPD-PCR analysis of enteropathogenic Escherichia coli (EPEC) clinical isolates.
Southeast Asian J Trop Med Public Health
;
2003 Sep; 34(3): 620-6
Article
in English
| IMSEAR
| ID: sea-35942
ABSTRACT
Enteropathogenic Escherichia coli (EPEC) is a leading cause of diarrhea among infants in developing countries. A total of 38 EPEC isolates, obtained from diarrhea patients of Hospital Miri, Sarawak, were investigated through plasmid profile, antibiotic resistance and randomly amplified polymorphic DNA (RAPD) analysis. From the 8 types of antibiotics used, all isolates were 100% resistant to furoxime, cephalothin and sulphamethoxazole and showed high multiple antibiotic resistant (MAR) indexes, ranging from 0.5 to 1.0. In plasmid profiling, 22 isolates (58%) showed the presence of one or more plasmids in the range 1.0 to 30.9 mDa. The dendrogram obtained from the results of the RAPD-PCR discriminated the isolates into 30 single isolates and 3 clusters at the level of 40% similarity. The EPEC isolates were highly diverse, as shown by their differing plasmid profiles, antibiotic resistance patterns and RAPD profiles.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plasmids
/
Humans
/
Drug Resistance
/
Microbial Sensitivity Tests
/
Predictive Value of Tests
/
DNA Fingerprinting
/
Random Amplified Polymorphic DNA Technique
/
Adult
/
Diarrhea
/
Escherichia coli
Type of study:
Evaluation studies
Country/Region as subject:
Asia
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS