Hemoglobin typing by high performance liquid chromatography.
Southeast Asian J Trop Med Public Health
;
1997 Jun; 28(2): 417-23
Article
in English
| IMSEAR
| ID: sea-35968
ABSTRACT
To evaluate the potential application of high performance liquid chromatography or HPLC in performing hemoglobin typing, comparison between this technique and routine methods was carried out. Blood specimens from Pediatric Hematology Unit, Research Center, Faculty of Medicine, Ramathibodi Hospital were examined by these methods. The level of Hb A2, Hb E and Hb F were compared. Hb A2 level determined by HPLC and electrophoresis was statistically significantly different but correlated well. Good correlation was found between Hb A2 level from HPLC compared with microcolumn chromatography although there was a statistical difference. No difference but very good correlation was found between the level of Hb E from HPLC compared with electrophoresis. Statistical difference was encountered when Hb F level determined by HPLC was compared to that determined by Betke alkaline denaturation test. However, good correlation was observed when the level of Hb F was greater than 2.0%. In conclusion, HPLC could be an alternative way of performing hemoglobin typing provided that Hb F is 10% or more, by calculating from the equation Alk F = (0.83 x Hb F by HPLC) - 0.98.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Protein Denaturation
/
Humans
/
Fetal Hemoglobin
/
Hemoglobin A2
/
Hemoglobin E
/
Hemoglobins
/
Blood Grouping and Crossmatching
/
Linear Models
/
Reproducibility of Results
/
Chromatography
Type of study:
Prognostic study
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1997
Type:
Article
Similar
MEDLINE
...
LILACS
LIS