Survival and infectivity of Brugia malayi microfilariae after cryopreservation.
Southeast Asian J Trop Med Public Health
;
1991 Jun; 22(2): 165-7
Article
in English
| IMSEAR
| ID: sea-36165
ABSTRACT
Methods were studied for the cryopreservation of microfilariae of periodic Brugia malayi. RPMI-1640 tissue culture medium containing 6% dimethyl sulfoxide (DMSO) and 15% newborn calf serum was used as cryoprotectant. Samples were frozen slowly in the vapor phase of liquid nitrogen prior to emersion in liquid nitrogen (-196 degrees C). The freezing rate was -0.5 to -1.0 degrees C per minute, microfilariae remained viable for as long as, 212 and 375 days, survival rates were 94 to 98% and they were infective to Aedes togoi mosquitos. The infective larvae (L3) were obtained for 10-11 days after feeding at 28 degrees C room-temperature and the infection rate of L3 in test mosquitos was 22.4-30.6%. All DMSO should be removed from the freezing medium to restore microfilariae activity after freezing.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Brugia
/
Humans
/
Chi-Square Distribution
/
Cryopreservation
/
Dimethyl Sulfoxide
/
Aedes
/
Animals
/
Microfilariae
/
Nitrogen
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1991
Type:
Article
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