Genetic variations and relationship among dengue virus type 3 strains isolated from patients with mild or severe form of dengue disease in Indonesia and Thailand.
Southeast Asian J Trop Med Public Health
;
2005 Sep; 36(5): 1187-97
Article
in English
| IMSEAR
| ID: sea-36175
ABSTRACT
Sequence analysis was conducted on structural and non-structural genes of 7 strains of dengue virus type-3 (DENV-3 virus) isolated in Indonesia and Thailand in the year 1973, 1994, and 1998 from patients with different clinical manifestations. In general, sequence similarity among isolates was greater than 93%, indicating that the mutation rate of DENV-3 circulating in this region was not more than 7% in the last 3 decades and suggesting that sequences that may responsible for viral architectures and/or biological function were strictly conserved. Mutations unique to viral strains associated with specific clinical manifestations were not found. Alignment of PrM/M and E nucleic acid sequences followed by parsimony analysis of sequences obtained in this study and published elsewhere allowed generation of phylogenetic trees, demonstrating that DENV-3 strains isolated in Indonesia in 1998 belonged to a separate cluster (subtype 2) from those isolated between 1973-1985 (subtype 1).
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Thailand
/
Genetic Variation
/
Humans
/
DNA, Viral
/
Molecular Sequence Data
/
Base Sequence
/
Sequence Homology, Nucleic Acid
/
Amino Acid Sequence
/
Sequence Homology, Amino Acid
/
Sequence Analysis, DNA
Country/Region as subject:
Asia
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2005
Type:
Article
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