Determination of insecticide susceptibility in Culex quinquefasciatus Say adults by rapid enzyme microassays.
Southeast Asian J Trop Med Public Health
;
1992 Sep; 23(3): 458-63
Article
in English
| IMSEAR
| ID: sea-36199
ABSTRACT
Rapid enzyme microassays for the detection of resistance due to organophosphate and carbamate in individual field-collected strains of Culex quinquefasciatus adults were conducted. These tests allowed accurate differentiation by eye, on the basis of color changes of susceptible and resistant individuals. Two separate tests were conducted for the biochemical assays. In the insensitive acetylcholinesterase (AChE) test, acetylthiocholine iodide (ACTH) and 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) were used as substrate and coupling agent respectively. The resulting yellow chromophore indicated AChE activity. Test results showed that the color intensity decreased as increasing concentrations of propoxur were added, thereby confirming the susceptibility of the enzyme to inhibitor. Assay of non-specific esterase however, indicated elevated levels which were correlated with degree of malathion resistance. Electrophoretic data revealed the presence of 2 esterase bands in all strains. It was concluded that such a pattern was not contributory to malathion resistance in adults.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Propoxur
/
Acetylcholinesterase
/
Time Factors
/
Biological Assay
/
Female
/
Insecticide Resistance
/
Culex
/
Dose-Response Relationship, Drug
/
Electrophoresis, Starch Gel
/
Animals
Country/Region as subject:
Asia
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
1992
Type:
Article
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