Comparison of the sensitive screening kit with two ELISA sets for detection of anti-Toxocara antibodies.
Southeast Asian J Trop Med Public Health
;
2000 Jun; 31(2): 394-8
Article
in English
| IMSEAR
| ID: sea-36210
ABSTRACT
Three serological tests (ELISA NOVUM, ELISA PU and ToxocaraCHEK) were compared using excretory-secretory (ES) antigen of second stage larvae of Toxocara canis on two sets of sera from patients aged 1-15 years, living in town and in the country, suspected of having larval toxocarosis. Of 60 serum samples examined for the presence of anti-Toxocara IgM and IgG antibodies 35 (58.3%) were positive with ELISA NOVUM, 30 (50.0%) with ELISA PU and 37 (61.6%) with ToxocaraCHEK. A significantly higher seropositivity with all the tests was detected in group of patients from the village (test chi2, p < 0.005) when compared with patients from the town. The highest sensitivity (100%) was detected for a rapid qualitative ToxocaraCHEK. Compared with this screening test, both ELISA sets showed higher specificity but a lower sensitivity. Consistent findings (+;-) with ELISA NOVUM and ELISA PU were 88.3%; ELISA NOVUM and ToxocaraCHEK 96.7% and ELISA PU and ToxocaraCHEK 86.7%. This comparison indicates the suitability of ToxocaraCHEK set for screening for its simplicity and rapidity in detecting anti-Toxocara antibodies.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Reagent Kits, Diagnostic
/
Toxocara
/
Humans
/
Immunoglobulin G
/
Immunoglobulin M
/
Enzyme-Linked Immunosorbent Assay
/
Antibodies, Helminth
/
Toxocariasis
/
Helminth Proteins
/
Child
Type of study:
Diagnostic study
/
Evaluation studies
/
Qualitative research
/
Screening study
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2000
Type:
Article
Similar
MEDLINE
...
LILACS
LIS