Preparation of Gnathostoma protein by ultrafiltration method using Nanosep membrane.
Southeast Asian J Trop Med Public Health
;
2003 ; 34 Suppl 2(): 121-2
Article
in English
| IMSEAR
| ID: sea-36342
ABSTRACT
We report our experience with Gnathostoma protein preparation by the ultrafiltration method. Crude antigen was sonicated and ultrafiltrated using the Nanosep 100 K membrane. SDS-PAGE electrophoresis showed protein bands at 43, 41, 24, 22, 21, 19.5 kDa. Use of the ultrafiltration method can provide specific protein (24 kDa), similar to the non-ultrafiltration method, with the other 5 non-specific proteins. Using the non-ultrafiltration method, there were more (20) non-specific protein. The ultrafiltration method can be an alternative method for the preparation of protein, which can provide better results than non-ultrafiltration.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Ultrafiltration
/
Helminth Proteins
/
Nanotechnology
/
Complex Mixtures
/
Gnathostoma
/
Animals
/
Membranes, Artificial
/
Antigens, Helminth
Language:
English
Journal:
Southeast Asian J Trop Med Public Health
Year:
2003
Type:
Article
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