Comparison of the protective efficacy on mekongi schistosomiasis in mice induced by antigens derived from cercariae, schistosomulae and adult worms.
Asian Pac J Allergy Immunol
;
1993 Jun; 11(1): 71-7
Article
in English
| IMSEAR
| ID: sea-36431
ABSTRACT
Protective efficacy of the extracts of cercariae, schistosomulae and adult worms of S. mekongi was studied in mice receiving immunizations with these extracts emulsified with Freund's complete adjuvant initially and incomplete adjuvant subsequently, and compared with mice receiving physiological saline with or without adjuvants as controls. After challenge with cercariae, the animals were sacrificed and the larvae or adult worms harvested by lung recovery and perfusion techniques on day 5 and weeks 6-8, respectively. Worm reduction rates were significantly higher in mice receiving extracts of schistosomula (59%) and adult worms (51%) than in those receiving the cercarial extracts (31%). Similar findings were obtained with the perfusion technique showing worm reduction rates of 57%, 53% and 30% in mice receiving extracts of schistosomulae, adult worms and cercariae, respectively. ELISA antibody titers were correspondingly increased in mice receiving extracts of schistosomulae and adult worms, but not in those receiving cercariae. This apparent association may be inadequate to suggest that the increase in ELISA titer be used as an indicator for resistance in mekongi schistosomiasis.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Schistosoma
/
Schistosomiasis
/
Female
/
Enzyme-Linked Immunosorbent Assay
/
Antibodies, Helminth
/
Freund's Adjuvant
/
Immunization
/
Disease Models, Animal
/
Animals
/
Mice
Type of study:
Prognostic study
Language:
English
Journal:
Asian Pac J Allergy Immunol
Year:
1993
Type:
Article
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