Immunohistochemical characterization of a new monoclonal antibody reactive with dengue virus-infected cells in frozen tissue using immunoperoxidase technique.
Asian Pac J Allergy Immunol
;
1996 Dec; 14(2): 107-13
Article
in English
| IMSEAR
| ID: sea-36473
ABSTRACT
This paper presents a novel monoclonal antibody shown to react with cytoplasmic antigens in various dengue infected human frozen organs from autopsy and necropsy specimens. Strong reactivity was found in hematopoietic cells, including immunoblasts, lymphocytes, plasma cells and macrophages of spleen, lymph node, lung, kidney and stomach. Strikingly, strong positivity was demonstrated in cerebral cortex neurones, Purkinje cells, choroid plexus and blood vessels in addition to astrocytes and microglia. Neurotropism of the virus could explain the meningitis, encephalitis, mononeuropathy and polyneuropathy observed by direct toxicity, but noted especially after an activation of mononuclear phagocytes and amplification of the immune response with subsequent vascular inflammation and formation of immune complexes.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Female
/
Humans
/
Male
/
Child, Preschool
/
Immunoenzyme Techniques
/
Adolescent
/
Dengue
/
Dengue Virus
/
Frozen Sections
/
Antibodies, Monoclonal
Language:
English
Journal:
Asian Pac J Allergy Immunol
Year:
1996
Type:
Article
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