A simple ELISA for antigiardial antibody using the membrane fraction of the parasite as antigen.
Asian Pac J Allergy Immunol
;
1985 Dec; 3(2): 183-6
Article
in English
| IMSEAR
| ID: sea-36555
ABSTRACT
Most existing methods for detecting antigiardia lamblia antibody require the use of cultured Giardia lamblia trophozoites as antigen in immunofluorescent and enzyme-linked immunosorbent assays. However, the maintenance of trophozoite culture systems limits the large-scale use of these antibody detection systems. An antigen extracted from Giardia lamblia by the detergent Triton X-100, when used in an ELISA system, produces specific, objective, quantitative and reproducible results. It is likely that such a test system could be packaged in kit form for large-scale diagnostic and epidemiological application in all parts of the world.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Enzyme-Linked Immunosorbent Assay
/
Fluorescent Antibody Technique
/
Giardiasis
/
Feces
/
Giardia
/
Animals
/
Antibodies
/
Antigens, Protozoan
/
Antigens, Surface
Language:
English
Journal:
Asian Pac J Allergy Immunol
Year:
1985
Type:
Article
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