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A simple ELISA for antigiardial antibody using the membrane fraction of the parasite as antigen.
Asian Pac J Allergy Immunol ; 1985 Dec; 3(2): 183-6
Article in English | IMSEAR | ID: sea-36555
ABSTRACT
Most existing methods for detecting antigiardia lamblia antibody require the use of cultured Giardia lamblia trophozoites as antigen in immunofluorescent and enzyme-linked immunosorbent assays. However, the maintenance of trophozoite culture systems limits the large-scale use of these antibody detection systems. An antigen extracted from Giardia lamblia by the detergent Triton X-100, when used in an ELISA system, produces specific, objective, quantitative and reproducible results. It is likely that such a test system could be packaged in kit form for large-scale diagnostic and epidemiological application in all parts of the world.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Humans / Enzyme-Linked Immunosorbent Assay / Fluorescent Antibody Technique / Giardiasis / Feces / Giardia / Animals / Antibodies / Antigens, Protozoan / Antigens, Surface Language: English Journal: Asian Pac J Allergy Immunol Year: 1985 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Humans / Enzyme-Linked Immunosorbent Assay / Fluorescent Antibody Technique / Giardiasis / Feces / Giardia / Animals / Antibodies / Antigens, Protozoan / Antigens, Surface Language: English Journal: Asian Pac J Allergy Immunol Year: 1985 Type: Article