Flow cytometric three-color determination of CD4 T-lymphocytes on blood specimens from AIDS patients who have a large number of contaminating non-lymphocytes.
Asian Pac J Allergy Immunol
;
1994 Dec; 12(2): 105-9
Article
in English
| IMSEAR
| ID: sea-36566
ABSTRACT
A three-color flow cytometric determination of CD4 T-lymphocytes on whole blood specimens from AIDS patients which contain a high proportion of non-lymphocyte elements is described. Peripheral blood cells were stained by a three-color method using monoclonal antibodies conjugated respectively with fluorescein isothiocyanate (FITC)-CD3, phycoerythrin (PE)-CD4 and peridinin chlorophyll protein (PerCP)-CD45. CD45 stains all leukocytes with the highest fluorescence expression of CD45 antigen in lymphocytes. By combining light scatter with CD45 in the fluorescence 3 (FL3) channel, a light scattering window can be drawn to include almost all bright CD45 lymphocytes. This live gate of lymphocytes was then acquired and analysed simultaneously using other irrelevant two-color (FITC/PE) antibodies of CD3 and CD4 in the FITC and PE channels, respectively. This method is easy and straightforward, and gives successful analysis of CD4 T-lymphocytes in AIDS blood specimens contaminated with an unusually large number of non-lymphocytic cells.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Male
/
CD4-Positive T-Lymphocytes
/
CD4 Antigens
/
Immunophenotyping
/
Acquired Immunodeficiency Syndrome
/
HIV Seropositivity
/
Leukocyte Common Antigens
/
CD3 Complex
/
CD4 Lymphocyte Count
Language:
English
Journal:
Asian Pac J Allergy Immunol
Year:
1994
Type:
Article
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