Production of specific monoclonal antibodies to Burkholderia pseudomallei and their diagnostic application.
Asian Pac J Allergy Immunol
;
1996 Jun; 14(1): 43-7
Article
in English
| IMSEAR
| ID: sea-36589
ABSTRACT
Hybridomas secreting monoclonal antibodies (MAbs) specific to Burkholderia pseudomallei were produced by immunizing BALB/cJ mice with crude culture filtrate of B. pseudomallei. Two monoclonal antibodies were found to be highly specific to B.pseudomallei as tested by indirect enzyme-linked immunosorbent assay and immunoblotting against a panel of crude whole cell extracts from B. pseudomallei, B. cepacia, Pseudomonas aeruginosa, P.putida, and Escherichia coli. One of the specific MAbs, clone SP-M, IgM subclass, could directly agglutinate all 42 B. pseudomallei, isolates. The study has shown that the agglutinating MAb has potential for rapid identification of B. pseudomallei in primary bacterial culture from clinical specimens. The antibody can be used in bacteriology laboratories to reduce time of biochemical methods, which require a few days.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rabbits
/
Enzyme-Linked Immunosorbent Assay
/
Burkholderia pseudomallei
/
Agglutination
/
Animals
/
Mice
/
Antibodies, Monoclonal
Type of study:
Prognostic study
Language:
English
Journal:
Asian Pac J Allergy Immunol
Year:
1996
Type:
Article
Similar
MEDLINE
...
LILACS
LIS