Use of endotoxin antigens in enzyme-linked immunosorbent assay for the diagnosis of P. pseudomallei infections (melioidosis).
Asian Pac J Allergy Immunol
;
1992 Dec; 10(2): 145-50
Article
in English
| IMSEAR
| ID: sea-36670
ABSTRACT
An enzyme-linked immunosorbent assay (ELISA) with endotoxin preparations of P. pseudomallei as antigen was developed for detection of IgG antibodies specific to melioidosis. Forty-seven sera of bacteriologically confirmed melioidosis patients, 55 non-melioidosis sera and 50 sera of healthy blood donors from non-endemic areas were subjected to this assay in comparison with indirect hemagglutination assay (IHA). The data were treated by receiver operating characteristics analysis. The sensitivity, specificity and accuracy in this ELISA were 95.7%, 94.2%, and 94.7%, respectively, with cut-off value of OD = 0.312 at 490 nm. Meanwhile, those in IHA were 81.0%, 91.4%, and 88.1%, respectively, with a cut-off value of > or = 1160. From these results, the ELISA was judged to be more reliable than IHA as the seroassay for diagnosis of melioidosis.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Blood Donors
/
Humans
/
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
Predictive Value of Tests
/
Reproducibility of Results
/
Sensitivity and Specificity
/
Burkholderia pseudomallei
/
Endotoxins
/
False Positive Reactions
Type of study:
Diagnostic study
/
Prognostic study
Language:
English
Journal:
Asian Pac J Allergy Immunol
Year:
1992
Type:
Article
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