Flow cytometric analysis of T-lymphocytes subsets in adult Thais.
Asian Pac J Allergy Immunol
;
1997 Sep; 15(3): 141-6
Article
in English
| IMSEAR
| ID: sea-36691
ABSTRACT
Flow cytometer (FACScan) was used to determine the range of T lymphocyte subpopulations in normal Thai blood donors at Maharaj Nakorn Chiang Mai Hospital, Chiang Mai. Reference population consisted of 150 healthy HIV seronegative blood donors. T lymphocyte subsets were analysed using two-color immunophenotyping of peripheral blood lymphocytes with a lysed whole blood technique and enumerated. The study showed that the normal values for CD3+ lymphocytes (percent), CD4+ lymphocytes (percent), CD8+ lymphocytes (percent), CD4/CD8 ratio, absolute CD3+ lymphocyte count, absolute CD4+ lymphocyte count and absolute CD8+ lymphocyte count were 64 +/- 8.8, 36.1 +/- 6.4, 25.7 +/- 7.3, 1.5 +/- 0.6, 1,630 +/- 600 cells/microl, 910 +/- 300 cells/microl and 670 +/- 350 cells/microl, respectively. We found that the values of CD3, CD4 and CD4/CD8 ratio were significantly lower than those in the Caucasians but those of CD8 was not significantly different. This observations have important clinical implication for the use of T lymphocyte subsets measurement, especially in the management of HIV infection in Thais. These normal ranges can be used as a reference for the decisions in clinical practice.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Reference Values
/
Thailand
/
Blood Donors
/
Humans
/
T-Lymphocyte Subsets
/
Age Factors
/
CD4-CD8 Ratio
/
Lymphocyte Count
/
Adult
/
Flow Cytometry
Type of study:
Prognostic study
Country/Region as subject:
Asia
Language:
English
Journal:
Asian Pac J Allergy Immunol
Year:
1997
Type:
Article
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