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Comparative study of respiratory syncytial virus in nasopharyngeal aspirates using conventional cell culture, shell viral centrifugation culture, immunofluorescence and biotin-avidin enzyme linked immunosorbent assays.
Asian Pac J Allergy Immunol ; 1991 Dec; 9(2): 121-4
Article in English | IMSEAR | ID: sea-37056
ABSTRACT
133 nasopharyngeal aspirates (NPA) were simultaneously tested for the presence of respiratory syncytial virus (RSV) by conventional cell culture (CCC), shell vial centrifugation culture (SVC), immunofluorescence assay (IFA) and biotin-avidin enzyme linked immunosorbent assay (B-A ELISA). These yielded positive results in 32(24%), 45(33.8%), 36(27%) and 40(30%) of specimens, respectively. Specimens positive by IFA and B-A ELISA were all also positive by SVC. The sensitivity of CCC, IFA, and B-A ELISA comparing to SVC was 71%, 80%, and 88.9%, respectively. For rapid detection of RSV, we recommend the SVC method where a cell culture laboratory is available and the B-A ELISA method where a cell culture laboratory is not available.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Respirovirus Infections / Respiratory Syncytial Viruses / Humans / Enzyme-Linked Immunosorbent Assay / Centrifugation / Nasopharynx / Fluorescent Antibody Technique / Sensitivity and Specificity Type of study: Diagnostic study Language: English Journal: Asian Pac J Allergy Immunol Year: 1991 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Respirovirus Infections / Respiratory Syncytial Viruses / Humans / Enzyme-Linked Immunosorbent Assay / Centrifugation / Nasopharynx / Fluorescent Antibody Technique / Sensitivity and Specificity Type of study: Diagnostic study Language: English Journal: Asian Pac J Allergy Immunol Year: 1991 Type: Article