One-step purification of chimeric green fluorescent protein providing metal-binding avidity and protease recognition sequence.

Prachayasittikul, Virapong; Isarankura Na Ayudhya, Chartchalerm; Piacham, Theeraphon; Kiatfuengfoo, Rachada

Prachayasittikul, Virapong; Isarankura Na Ayudhya, Chartchalerm; Piacham, Theeraphon; Kiatfuengfoo, Rachada.

Asian Pac J Allergy Immunol ; 2003 Dec; 21(4): 259-67

Article in En | IMSEAR | ID: sea-37082

Subject(s)

Binding Sites; Burkholderia pseudomallei/enzymology; Chromatography, Affinity/methods; Electrophoresis, Polyacrylamide Gel; Enteropeptidase/metabolism; Genetic Engineering; Green Fluorescent Proteins/chemistry; Histidine/metabolism; Metals/metabolism; Peptide Hydrolases/metabolism; Recombinant Fusion Proteins/chemistry; Zinc/metabolism

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Full text: 1 Index: IMSEAR Main subject: Peptide Hydrolases / Zinc / Binding Sites / Recombinant Fusion Proteins / Enteropeptidase / Genetic Engineering / Chromatography, Affinity / Burkholderia pseudomallei / Green Fluorescent Proteins / Electrophoresis, Polyacrylamide Gel Type of study: Evaluation_studies Language: En Journal: Asian Pac J Allergy Immunol Year: 2003 Type: Article

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