Evaluation of biotin-streptavidin enzyme-linked immunosorbent assay for detection of genital herpes simplex virus infection.
Asian Pac J Allergy Immunol
;
1987 Dec; 5(2): 143-8
Article
in English
| IMSEAR
| ID: sea-37105
ABSTRACT
A biotin-streptavidin enzyme-linked immunosorbent assay (B-SA ELISA) was evaluated for detection of herpes simplex virus (HSV) in clinical specimens which were cervico-vaginal swabs from 205 asymptomatic women and swabs from the genital lesions of 163 suspected patients. All specimens were also subjected to a conventional virus isolation in cell culture. A blocking B-SA ELISA had 100% specificity and 98% sensitivity compared with viral isolation from patients, but had only 40% sensitivity using specimens from asymptomatics. The conventional B-SA ELISA might also be used; it gave results corresponding to B-SA ELISA blocking test except for a single specimen which was considered a false positive.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Bacterial Proteins
/
Vaginal Smears
/
Biotin
/
Female
/
Humans
/
Male
/
Enzyme-Linked Immunosorbent Assay
/
Herpes Genitalis
/
Predictive Value of Tests
/
Simplexvirus
Type of study:
Diagnostic study
/
Prognostic study
Language:
English
Journal:
Asian Pac J Allergy Immunol
Year:
1987
Type:
Article
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