Retrospective study on the diagnostic value of IgG ELISA, dot immunoassay and indirect hemagglutination in septicemic melioidosis.
Asian Pac J Allergy Immunol
;
2001 Jun; 19(2): 129-33
Article
in English
| IMSEAR
| ID: sea-37222
ABSTRACT
Three serological methods for diagnosis of melioidosis were compared with the culture method currently used as the "gold standard". The diagnostic values of the serological methods were evaluated retrospectively in 306 patients residing in an endemic area. The enzyme-linked immunosorbent assay (ELISA), using affinity purified antigen for detecting specific IgG antibody, showed a slightly higher specificity (86.0%) than the dot immunoassay (DOT) (84.0%) and both were superior to indirect hemagglutination (IHA) (72.0%). The sensitivity of DOT (96.4%) and ELISA (85.7%) were considerably higher than that of IHA (50.0%). The primary benefit of the high negative predictive value of both ELISA (96.4%) and DOT (99.0%) in an area of high prevalence is the ability to rule out most of the non-melioidosis patients.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Thailand
/
Humans
/
Immunoglobulin G
/
Enzyme-Linked Immunosorbent Assay
/
Hemagglutination Tests
/
Immunoblotting
/
Predictive Value of Tests
/
Retrospective Studies
/
Sensitivity and Specificity
/
Burkholderia pseudomallei
Type of study:
Diagnostic study
/
Evaluation studies
/
Observational study
/
Risk factors
Country/Region as subject:
Asia
Language:
English
Journal:
Asian Pac J Allergy Immunol
Year:
2001
Type:
Article
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