Quantitation of human immunodeficiency virus type 1 DNA by competitive nested PCR assay.
Asian Pac J Allergy Immunol
;
2001 Mar; 19(1): 43-8
Article
in English
| IMSEAR
| ID: sea-37225
ABSTRACT
A quantitative competitive nested PCR assay was developed for quantifying HIV-1 proviral DNA in clinical samples. A competitor DNA was constructed from a conserved region of the HIV-1 gag gene by deleting a sequence of 18 base pairs. We quantitated HIV-1 proviral DNA copy number in clinical samples. Peripheral blood mononuclear cells (PBMCs) from 35 HIV-infected patients with a CD4 count range of 4-728 cell/mm3 were analyzed by this method. The copy numbers of HIV-1 DNA detected ranged between 518 to 67,340 copies per 10(6) CD4+ T-cells. The copy numbers correlated inversely with the CD4 counts.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Thailand
/
Humans
/
DNA, Viral
/
CD4-Positive T-Lymphocytes
/
HIV Infections
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
HIV-1
/
CD4 Lymphocyte Count
/
Endpoint Determination
Type of study:
Diagnostic study
/
Evaluation studies
Country/Region as subject:
Asia
Language:
English
Journal:
Asian Pac J Allergy Immunol
Year:
2001
Type:
Article
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