Development of an enzyme-linked immunosorbent assay for detection of IgE antibodies specific to Dermatophagoides pteronyssinus.
Asian Pac J Allergy Immunol
;
1988 Dec; 6(2): 103-6
Article
in English
| IMSEAR
| ID: sea-37248
ABSTRACT
Allergy to Dermatophagoides pteronyssinus was determined in 61 rhinitis patients using prick test (PT), enzyme-immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). A total of 43 patients tested positive with PT. Forty six patients were positive when tested with EIA and ELISA. With PT as standard test, EIA was found to have 83.7% sensitivity and 44.4% specificity; ELISA had 81.4% sensitivity and 38.9% specificity. There was a linear relationship between absorbance values obtained by EIA and ELISA. The performance time was 8 hours, 24 hours and 30 minutes for ELISA, EIA and PT respectively. The cost per test for ELISA, EIA and PT was US$ 0.20, US$ 5.20 and US$ 0.14 respectively. It was concluded that ELISA was more cost-effective than EIA should be used to supplement PT for a more complete diagnosis of allergy.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Immunoglobulin E
/
Enzyme-Linked Immunosorbent Assay
/
Skin Tests
/
Immunoenzyme Techniques
/
Sensitivity and Specificity
/
Cost-Benefit Analysis
/
Hypersensitivity
/
Animals
/
Malaysia
Type of study:
Diagnostic study
Country/Region as subject:
Asia
Language:
English
Journal:
Asian Pac J Allergy Immunol
Year:
1988
Type:
Article
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