Karyotyping in retinoblastoma--a statistical approach.
Article
in English
| IMSEAR
| ID: sea-37624
ABSTRACT
PURPOSE:
Karyotype analysis in hereditary retinoblastoma is considered to be of marginal value in risk prediction due to uncertainties in the assessment of 13q14 deletions. However, it is a low cost genetic test for retinoblastoma in developing countries. In the present study, the results of karyotype analysis were refined by a statistical method to overcome limitations.METHODS:
Karyotype analysis was performed by trypsin-Giemsa banding and naked eye karyotyping for 33 bilateral, 25 unilateral and one regressed retinoblastoma patients. The percentage of metaphases with 13q14 deletions in each case was plotted on a scatter diagram. Normalization of the data was achieved by log transformation and the results were statistically analyzed by one-sample 't' test using SPSS version 9.0.RESULTS:
Seven samples had 13q14 deletion percentages above the cutoff value. One-sample 't' test showed significance (p< 0.001). By this method, two unilateral and five bilateral patients had 13q14 deletions, constituting 11.8 % of cases.CONCLUSION:
For accuracy, statistical analysis should be considered as an adjunct in karyotyping.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Retinoblastoma
/
Chromosomes, Human, Pair 13
/
Humans
/
Statistics as Topic
/
Chromosome Banding
/
Chromosome Deletion
/
Retinal Neoplasms
/
Karyotyping
Type of study:
Prognostic study
Language:
English
Year:
2005
Type:
Article
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