Acridine orange staining and viability of the coccoid form of Campylobacter upsaliensis.
Article
in English
| IMSEAR
| ID: sea-38369
ABSTRACT
Conversion of Campylobacter upsaliensis to the coccoid form during aerobic incubation at 37 degrees C was not prevented by treatment with chloramphenicol and was accompanied by severe decreases in isocitrate dehydrogenase activity and oxygen uptake. Although the coccoid forms fluoresced orange-red by acridine orange staining, agarose gel electrophoresis indicated an extensive degradation of the ribosomal RNA. This suggests that acridine orange staining may not be a good indicator of viability and that the coccoid form of C. upsaliensis at 37 degrees C is degenerative rather than part of the life cycle.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Oxygen Consumption
/
Acridine Orange
/
RNA, Ribosomal
/
Campylobacter upsaliensis
/
Microbial Viability
/
Fluorescent Dyes
/
Isocitrate Dehydrogenase
Language:
English
Year:
2005
Type:
Article
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