BCR/ABL rearrangement in Philadelphia chromosome negative CML patients.
Article
in English
| IMSEAR
| ID: sea-39275
ABSTRACT
Reverse transcription-polymerase chain reaction (RT-PCR) was used to study 34 patients with chronic myelogenous leukemia (CML) associated with negative Philadelphia (Ph) chromosome. This report showed evidence of a chimeric BCR/ABL transcript in 18 (52.9%) and 28 (82.4%) cases by first PCR and seminested PCR, respectively. In these BCR/ABL transcript positive cases, the incidence of BCR exon3/ABL exon2 (B3A2) and BCR exon 2/ABL exon2 rearrangement was 25 (89.3%) and 3 (10.7%) cases, respectively. The other 6 Ph negative patients showed no evidence of reciprocal translocation of BCR to chromosome 9. This data demonstrates that seminested PCR is sufficiently sensitive to detect BCR/ABL fusion transcript in Ph chromosome negative CML patients.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Reference Values
/
Philadelphia Chromosome
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Female
/
Humans
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Male
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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Gene Amplification
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Fusion Proteins, bcr-abl
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Sensitivity and Specificity
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Reverse Transcriptase Polymerase Chain Reaction
Type of study:
Diagnostic study
Language:
English
Year:
2000
Type:
Article
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