Growth inhibition of breast cancer cell line MCF-7 by siRNA silencing of Wilms tumor 1 gene.
Article
in English
| IMSEAR
| ID: sea-40149
ABSTRACT
BACKGROUND:
RNA interference (RNAi) is sequence-specific inhibition of gene expression induced by double-stranded RNA.OBJECTIVE:
Define the role of Wilms tumor 1 gene (WT1) in breast cancer oncogenesis using RNAi. MATERIAL ANDMETHOD:
MCF-7 breast cancer cells, which express a high level of WT1, were transfected with synthetic small interfering RNA (siRNA) targeting WT1 (siRNA(WT1)) resulting in inhibition of WTI expression, as well as growth, in a dose- and time-dependent manner.RESULT:
The minimum concentration of siRNA(WT1) for growth inhibition and WT1 silencing was 25 nM and 50 nM respectively. WT1 expression was completely abolished at 200 nM siRNA,.CONCLUSION:
These data suggest that WTI1is indispensable for the survival of breast cancer MCF-7 cell line.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Breast Neoplasms
/
Female
/
Humans
/
Gene Expression
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Wilms Tumor
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Proto-Oncogene Proteins c-bcl-2
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Gene Silencing
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RNA, Small Interfering
/
RNA Interference
/
Cell Line, Tumor
Language:
English
Year:
2007
Type:
Article
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