Vitrification of mouse oocyte using open pulled straws compared with needles.
Article
in English
| IMSEAR
| ID: sea-40522
ABSTRACT
OBJECTIVE:
To compare the survival rate of mouse oocytes and fertilization rate between using open pulled straws (OPS) and needles for vitrification. MATERIAL ANDMETHOD:
Meiosis II oocytes from female C57B/6J mice aged 7-8 weeks were collected and allocated to two groups for vitrification by using OPS or needles. Vitrified oocytes were thawed, morphological survival and fertilization rate were examined.RESULTS:
There was no obvious difference between the morphological survival rates of vitrified mouse oocytes using OPS and needles (66.7% vs 64.8%). Proportions Difference 1.9% (95% CI -7.1, 10.7). The vitrified oocytes from the needle had significantly higher percentages of fertilization rate than OPS (76.8% vs 62.5%). Proportions Difference -14.3% (95% CI -24.5, -3.6).CONCLUSION:
Vitrification method of mouse oocytes using needles when compared to OPS provides a similar morphological survival rate and higher fertilization rate.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Oocytes
/
Female
/
Cryopreservation
/
Animals
/
Meiosis
/
Mice
/
Mice, Inbred C57BL
/
Needles
Language:
English
Year:
2006
Type:
Article
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