The preservation method and timing on accuracy of manual leukocytes counts in synovial fluid.
Article
in English
| IMSEAR
| ID: sea-42629
ABSTRACT
OBJECTIVES:
To compare the efficacy of heparin and EDTA and determine the impact of time delays in stabilizing leukocyte counts in synovial fluid. MATERIAL ANDMETHOD:
33 specimens were collected in heparin-preserved and EDTA-preserved containers. Total cell count was performed manually at 1 hour and 24 hours. Correlation between cell counts from both preservatives and the leukocyte number at 1 hour and 24 hours were analyzed by means of agreement measurement.RESULTS:
There were good correlations between the leukocyte numbers from the specimens preserved by heparin and EDTA (ICC = 0.889, r = 0.879, P < 0.0001 at 1 hour and ICC = 0.822, r = 0.693, p < 0.0001 at 24 hour). At 24 hours, total cell counts from EDTA-preserved samples were comparable to those obtained at 1 hour (ICC = 0.985, r = 0. 986, p < 0. 0001) and were not different from those of the heparinized samples (ICC = 0.833, r = 0. 751, p < 0.0001) but the ICC value was higher.CONCLUSION:
EDTA was as effective as heparin for preservation of synovial fluid. Therefore, it can be used routinely as a preservative of synovial fluid.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Preservation, Biological
/
Synovial Fluid
/
Time Factors
/
Female
/
Humans
/
Male
/
Heparin
/
Cross-Sectional Studies
/
Edetic Acid
/
Leukocytes
Type of study:
Observational study
/
Prevalence study
/
Risk factors
Language:
English
Year:
2006
Type:
Article
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