Detection of pathogenic leptospiral DNA in urine by polymerase chain reaction.
Article
in English
| IMSEAR
| ID: sea-42732
ABSTRACT
Diagnosis of leptospirosis is currently based on serological tests detecting antibodies against the spirochete. The standard method is the microscopic agglutination test (MAT), which is serovar-specific, requires a period of antibody development, and increases the risk of exposure to viable organisms. Therefore, the present study aimed to develop a rapid, sensitive, specific, and safe method based on the PCR technique to detect pathogenic Leptospira in urine samples. Nested PCR using two sets of primers, external and internal primers, were shown to specifically amplify 16S rRNA target of patho-genic Leptospira. No amplification was observed when DNA from non-pathogenic Leptospira and other non-Leptospira bacteria were used as DNA templates. The method was able to detect as few as 10 leptospires in urine. Therefore, nested PCR approach may be a useful tool for prompt and definitive diagnosis of leptospirosis.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Urine
/
Humans
/
Agglutination Tests
/
Polymerase Chain Reaction
/
Sensitivity and Specificity
/
Leptospira
Type of study:
Diagnostic study
Language:
English
Year:
2004
Type:
Article
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