Cryodamage on sperm chromatin according to different freezing methods, assessed by AO test.
Article
in English
| IMSEAR
| ID: sea-43110
ABSTRACT
OBJECTIVE:
To evaluate cryodamage effects on human sperm chromatin, motility and cryosurvival rate after freeze-thawing, compared between liquid nitrogen vapour and computerized program freezer, assessed by acridine orange staining method (AO test). MATERIAL ANDMETHOD:
Fifty semen samples were used. After semen analysis, each semen sample was mixed with cryoprotective media and divided into 2 straws. The first straw was frozen with liquid nitrogen vapour and the second with computerized program freezer. After 1 month of cryostorage, semen samples were thawed. Sperm chromatin integrity, motility, morphology, vitality and sperm cryosurvival rate were determined.RESULTS:
DNA damage was significantly greater (p < 0.001) following freezing with liquid nitrogen vapour than with computerized program freezer. Furthermore, the computerized program freezing method significantly provided superior post-thaw sperm motility, vitality and cryosurvival rate, compared with the liquid nitrogen vapour freezing method.CONCLUSION:
Computerized program freezing is recommended as a first choice method for routine cryostorage.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Semen Preservation
/
Sperm Motility
/
Spermatozoa
/
Acridine Orange
/
DNA Damage
/
Humans
/
Male
/
Computers
/
Chromatin
/
Cryopreservation
Language:
English
Year:
2006
Type:
Article
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