Tissue engineering of cartilage with porous polycarprolactone--alginate scaffold: the first report of tissue engineering in Thailand.
Article
in English
| IMSEAR
| ID: sea-43430
ABSTRACT
OBJECTIVE:
To engineer human cartilage with porous polycaprolactone (PCL)-Alginate Scaffold.BACKGROUND:
Polycaprolactone (PCL) is a prolonged degradable polymer that has good mechanical strength. The authors fabricated PCL as an ear shaped scaffold. Alginate hydrogel was used to seed chondrocyte into the PCL porous scaffold by a gel-cell seeding technique. MATERIAL ANDMETHOD:
PCL Scaffolds were fabricated like human pinna by particle leaching technique. Chondrocyte was isolated from human rib cartilage and then cultured. The cultured chondrocyte were mixed with 1.2% alginate and b-FGF (basic-fibroblast growth factor) 5 ng/ml at a concentration of 25 x 10(6) cell/ml, then were seeded in porous PCL scaffold to make the constructs. The constructs were cultured in vitro for 1 week. Then they were implanted in subcutaneous plane of the back of six-female nude mice (5 weeks old). Two nude mice were sacrificed at 2, 3, and 6 months. Histological study was done (H&E, Alcian blue, collagen type II).RESULT:
Neocartilage was formed in the porous cavity of PCL scaffold. At 2 and 3 months, neocartilage were similar to very young cartilage. At 6 months, they were mature. The delayed maturation until 6 months and the highly vascularization of neocartilage in the early phase was the effect of human b-FGF The growths of neocartilage islands in porous cavity were also observed along with degradation ofPCL inter-porous septum.CONCLUSION:
This paper reports the first success of cartilage tissue engineering in Thailand.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Polyesters
/
Thailand
/
Female
/
Cartilage
/
Cells, Cultured
/
Porosity
/
Chondrocytes
/
Tissue Engineering
/
Alginates
/
Animals
Country/Region as subject:
Asia
Language:
English
Year:
2006
Type:
Article
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