Shedding of HIV-1 subtype E in semen and cervico-vaginal fluid.
Article
in English
| IMSEAR
| ID: sea-43835
ABSTRACT
The uneven expansion of HIV-1 subtypes in each transmitted group raises the possibility that some viruses have less/more potential by qualitative/quantitative for heterosexual transmission compared to others. In Thailand, HIV-1 subtype E is mainly spread via heterosexual route and accounts for about 95 per cent of the infected cases. To determine whether high sexual infectivity of HIV-1 subtype E is due to the presence of a virus in genital fluid, we conducted a study to characterize shedding of HIV-1 in seminal and cervico-vaginal fluids of 30 HIV-1 subtype E infected Thai couples by PCR and virus isolation methods. All subjects had no HIV-associated diseases and other sexually transmitted diseases. HIV-1 subtype E DNA was detected in 22/30 (77.33%) of cervico-vaginal and also 22/30 (77.33%) of seminal fluid samples. The isolation rate of HIV-1 from semen and cervico-vaginal secretion was 36.67 per cent and 16.67 per cent, respectively. Number of HIV-1 subtype E DNA copies in the blood is reversely correlated with the number of blood CD4+ T cells, while that in genital fluid was not related to CD4+ T cell count. An increase in shedding of HIV- DNA subtype E in female genital tract compared to other HIV subtypes reported by other investigators might be one reason to explain the rapid spread of subtype E by heterosexual transmission in Thailand.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Thailand
/
Vagina
/
Female
/
Humans
/
Male
/
DNA, Viral
/
Sexually Transmitted Diseases, Viral
/
HIV Infections
/
Cervix Uteri
/
Polymerase Chain Reaction
Type of study:
Qualitative research
Country/Region as subject:
Asia
Language:
English
Year:
1997
Type:
Article
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