Comparative evaluation of hexokinase isoenzyme typing and antigen detection for differentiation of Entamoeba histolytica and Entamoeba dispar.
Article
in English
| IMSEAR
| ID: sea-46649
ABSTRACT
Entamoeba histolytica, the causative organism of invasive amebiasis is a potential pathogen, while asymptomatic infection is caused by E. dispar. Differentiation of the species is not possible on the basis of morphological characters by microscopic examination. In the present study an attempt has been made to differentiate E. histolytica from E. dispar in 45 isolates obtained from culture and direct stool samples respectively on the basis of hexokinase isoenzyme analysis and Tech Lab ELISA. A 100% correlation was found between these two techniques. However, Tech Lab E. histolytica antigen detection test was found to be both rapid and technically simple. Its use in diagnosis and epidemiological studies is recommended.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Enzyme-Linked Immunosorbent Assay
/
Entamoeba
/
Entamoeba histolytica
/
Entamoebiasis
/
Hexokinase
/
Isoenzymes
/
Animals
/
Antigens, Protozoan
Type of study:
Diagnostic study
Language:
English
Year:
2004
Type:
Article
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