Reverse line probe assay for the rapid detection of rifampicin resistance in Mycobacterium leprae.
Article
in English
| IMSEAR
| ID: sea-46690
ABSTRACT
Mutations in the rpoB gene of 40 biopsy isolates of Mycobacterium leprae were analyzed by reverse hybridization-based line probe assay after PCR, and nine distinct single-nucleotide substitutions were found. Among them, a 3-nucleotide substitution was found in two, and 2-nucleotide substitutions were found in seven isolates. This is a new finding of multiple mutations in a single point of the rpoB gene for rifampicin resistance. This investigation demonstrates that the pattern of mutations in the rpoB gene for rifampicin resistance in Nepal involves more variety.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rifampin
/
Biological Assay
/
Biopsy
/
Humans
/
Polymerase Chain Reaction
/
Drug Resistance, Bacterial
/
Genes, Bacterial
/
Leprostatic Agents
/
Leprosy
/
Mycobacterium leprae
Type of study:
Diagnostic study
Language:
English
Year:
2006
Type:
Article
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