A study on cryopreservation of cultured rabbit periodontal ligament cells.
Article
in English
| IMSEAR
| ID: sea-51886
ABSTRACT
Cultured rabbit periodontal ligament cells were subjected to short term cryopreservation in liquid nitrogen, for a period of 84 hours and 168 hours, to study the effect of cryopreservation on coll viability and culturing ability. The vital cell count performed by Trypan Blue exclusion was 14.93 x 10(0) cells/ml in vial A and 9.11 x 10(6) cell/ml in vial B, before cryopreservation. The loss of viability was minimal--vital cell count being 14.64 x 10(6) cells/ml in vial A and 8.87 x 10(6) cells/ml in vial B after 84 hrs of cryopreservation and 14.6 x 10(6) cells/ml in vial A and 8.82 x 10(6) cells/ml in vial B after 168 hours of cryopreservation. The cryopreserved cells after thawing could grow again in cultured.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Periodontal Ligament
/
Rabbits
/
Time Factors
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Trypan Blue
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Female
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Male
/
Cryopreservation
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Cell Adhesion
/
Cell Count
/
Cell Division
Language:
English
Year:
2002
Type:
Article
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