Comparison of the mouse foot pad test with a Buddemeyer type radiorespirometric assay in detecting viable Mycobacterium leprae in human lesional biopsies.
Indian J Dermatol Venereol Leprol
;
2007 Nov-Dec; 73(6): 384-8
Article
in English
| IMSEAR
| ID: sea-52149
ABSTRACT
AIM:
This study assesses the utility of a Buddemeyer type radiorespirometric (RR) assay in detecting viable Mycobacterium leprae in clinical samples taking the mouse foot pad (MFP) test as gold standard.METHODS:
A total of 131 skin biopsy specimens comprising of 56 untreated, smear-positive, borderline lepromatous and lepromatous leprosy (BL-LL) and 75 untreated, smear-negative, borderline tuberculoid and mid-borderline (BT-BB) specimens were processed by both the methods. The cut-off value (in counts per minute, cpm) for test samples in the RR assay was determined using nonleprous (normal) skin biopsy specimens.RESULTS:
In the untreated BL-LL and BT-BB groups, 86 and 56% of the cases tested positive in the RR assay respectively, which was comparable to the results of the MFP test (80 and 45% respectively). The overall concordance between the two tests was 74.79%, whereas the sensitivity and specificity were 75 and 74.3% respectively. A Kappa value of 0.459 indicated a fairly good agreement between the two methods. There was no linear relationship observed between the bacteriological index (BI) and the morphological index (MI) with the counts per minute (cpm) output.CONCLUSION:
The results indicate the ability of this RR assay to detect viability; however a comparison with another sensitive method would further validate the assay system.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Skin
/
Biopsy
/
Humans
/
Microbial Sensitivity Tests
/
Sensitivity and Specificity
/
Leprosy
/
Animals
/
Mice
/
Mycobacterium leprae
Type of study:
Diagnostic study
Language:
English
Journal:
Indian J Dermatol Venereol Leprol
Year:
2007
Type:
Article
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