Detection of vancomycin resistant Staphylococcus aureus: a comparative study of three different phenotypic screening methods.
Indian J Med Microbiol
;
2005 Jan; 23(1): 52-5
Article
in English
| IMSEAR
| ID: sea-53592
ABSTRACT
The objective of this study was to investigate screening methodologies, to detect Staphylococcus aureus strains with decreased susceptibility to vancomycin. Three methods were used to screen 160 Staphylococcus aureus clinical isolates along with ATCC quality control strains. Subsequently, MIC of all these 160 strains were determined by NCCLS methodology. The MIC of all the 160 clinical isolates was < or = 4 microg/mL and were classified as vancomycin susceptible by NCCLS criteria but 23 strains were positive by Hiramatshu method, two grew on MHA (5 microg/mL vancomycin) while CDC method correctly identified no vancomycin intermediate S.aureus (VISA) or vancomycin resistant S.aureus (VRSA) strains with reference to there MIC. CDC method was found to be the most appropriate screening methodology for detection of VISA or VRSA for diagnostic laboratories.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Phenotype
/
Staphylococcal Infections
/
Staphylococcus aureus
/
Microbial Sensitivity Tests
/
Bacteriological Techniques
/
Vancomycin Resistance
Type of study:
Diagnostic study
/
Prognostic study
/
Screening study
Language:
English
Journal:
Indian J Med Microbiol
Journal subject:
Microbiology
Year:
2005
Type:
Article
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