Chlamydia trachomatis antigen detection in pregnancy and its verification by antibody blocking assay.
Indian J Med Microbiol
;
2006 Apr; 24(2): 97-100
Article
in English
| IMSEAR
| ID: sea-53774
ABSTRACT
PURPOSE:
To detect the prevalence of genital infection caused by Chlamydia trachomatis in pregnant women and also to confirm the positive results using blocking antibody assay.METHODS:
Endocervical specimens were collected from 200 symptomatic and asymptomatic pregnant women attending the ANC OPD at M P Shah Medical College, Jamnagar. The samples were tested for presence of Chlamydia trachomatis antigen using the monoclonal antibody. Blocking antibody assay was used to further verify the positive results.RESULTS:
Out of 200 pregnant women, 38 (19%) were found positive for Chlamydia trachomatis antigen. Out of the 68 symptomatic patients, C. trachomatis antigen was detected in 26.4%. After verification of the positive samples 13.6% of the asymptomatic pregnant women were found to be harbouring the infection in their genital tract. Two (5.2%) out of the 38 positive samples, on verification with the blocking antibody assay, were found to be false positive by IDEIA,TM thus the specificity of the IDEIATM being 94.8%. In patients with previous history of abortions, 27.7% were tested positive for C. trachomatis infection.CONCLUSIONS:
Significant number of pregnant women shad C. trachomatis antigen in their endocervical canal, which can be easily diagnosed by this simple enzyme immuno assay having a specificity of 94.8%. Verification of positive results by antibody blocking assay can further improve the specificity of this non-culture test. Asymptomatic patients should also be screened for the infection. History of previous abortions places the patient at a higher risk for C. trachomatis infection thus such patients should be definitely tested for chlamydia infection.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Pregnancy Complications, Infectious
/
Reagent Kits, Diagnostic
/
Female
/
Humans
/
Pregnancy
/
Immunoassay
/
Chlamydia Infections
/
Cervix Uteri
/
Chlamydia trachomatis
/
Incidence
Type of study:
Diagnostic study
/
Incidence study
/
Prognostic study
Language:
English
Journal:
Indian J Med Microbiol
Journal subject:
Microbiology
Year:
2006
Type:
Article
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