Dedicated decontamination: A necessity to prevent cross contamination in high throughput mycobacteriology laboratories.
Indian J Med Microbiol
;
2007 Jan; 25(1): 4-6
Article
in English
| IMSEAR
| ID: sea-53859
ABSTRACT
Unrecognized cross-contamination has been known to occur in laboratories frequently, especially with sensitive recovery system like BACTEC 460 TB. In March 2001, we investigated a pseudo-outbreak of Mycobacterium tuberculosis isolates in three smear negative clinical specimens and would like to present our experience in this communication. METHODS:
All suspected cases were confirmed by checking the drug susceptibility and DNA fingerprints using spoligotyping as well as restriction fragment length polymorphism.RESULTS:
On investigation, the most likely cause was found to be the use of common decontamination reagents and phosphate buffer.CONCLUSIONS:
To avoid erroneous diagnosis, we have devised a dedicated decontamination procedure, which includes separate aliquoting of phosphate buffer and decontamination reagents per patient. Timely molecular analysis and appropriate changes to specimen processing have been identified as useful measures for limiting laboratory cross contamination.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Specimen Handling
/
Polymorphism, Restriction Fragment Length
/
Humans
/
DNA, Bacterial
/
Equipment Contamination
/
Retrospective Studies
/
DNA Fingerprinting
/
Bacteriological Techniques
/
Clinical Laboratory Techniques
/
Diagnostic Errors
Type of study:
Diagnostic study
/
Observational study
Language:
English
Journal:
Indian J Med Microbiol
Journal subject:
Microbiology
Year:
2007
Type:
Article
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