SHV-5 extended-spectrum beta-lactamases in clinical isolates of Escherichia coli in Malaysia.
Indian J Med Microbiol
;
2006 Jul; 24(3): 205-7
Article
in English
| IMSEAR
| ID: sea-53929
ABSTRACT
Escherichia coli isolates resistant to ceftazidime isolated in the University Malaya Medical Center (UMMC) Kuala Lumpur, Malaysia, between the years 1998 and 2000 were studied for extended-spectrum beta-lactamase (ESBL) production. All strains were analysed phenotypically and genotypically and found to be ESBL-producing organisms harbouring SHV-5 beta-lactamase. This was confirmed by PCR-SSCP and nucleotide sequencing of the blaSHV amplified gene. As there was no evidence of ESBL activity in E. coli prior to this, coupled with the fact that there was a predominance of SHV-5 beta-lactamases in Klebsiella pneumoniae isolates in UMMC, we postulate that the E. coli obtained the SHV-5 beta-lactamase genes by plasmid transfer from the ESBL-producing K. pneumoniae.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Phenotype
/
Beta-Lactamases
/
Humans
/
DNA, Bacterial
/
Microbial Sensitivity Tests
/
Ceftazidime
/
Polymerase Chain Reaction
/
Polymorphism, Single-Stranded Conformational
/
Beta-Lactam Resistance
/
Gene Transfer, Horizontal
Country/Region as subject:
Asia
Language:
English
Journal:
Indian J Med Microbiol
Journal subject:
Microbiology
Year:
2006
Type:
Article
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