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Human anti-rhinosporidial antibody does not cause metabolic inactivation or morphological damage in endospores of Rhinosporidium seeberi, in vitro.
Indian J Med Microbiol ; 2005 Jan; 23(1): 14-9
Article in English | IMSEAR | ID: sea-54017
ABSTRACT
This report describes the use of the MTT-reduction and Evan's blue-staining tests for the assessment of the viability and morphological integrity, respectively, of rhinosporidial endospores after exposure to sera from rhinosporidial patients with high titres of anti-rhinosporidial antibody. Sera from three patients, with nasal, ocular and disseminated rhinosporidiosis respectively were used, with human serum without anti-rhinosporidial antibody for comparison, with or without added fresh guinea pig serum as a source of complement. All four sera tested, with or without guinea-pig serum, had no effect on the morphological integrity or the viability of the endospores and it is suggested that anti-rhinosporidial antibody has no direct protective role against the endospores, the infective stage, in rhinosporidiosis. This finding is compatible with the occurrence of chronicity, recurrence and dissemination that are characteristic of rhinosporidiosis despite the presence of high titres of anti-rhinosporidial antibody in patients with these clinical characteristics. The possible occurrence of humoral mechanisms of immunity that involve anti-rhinosporidial antibody with cells such as leucocytes and NK cells, in vivo, cannot yet be discounted, although the presence of high titres of anti-rhinosporidial antibody in patients with chronic, recurrent and disseminated lesions might indicate that such antibody is non-protective in vivo.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Rhinosporidiosis / Rhinosporidium / Spores, Fungal / Staining and Labeling / Humans / Antibodies, Fungal / Antigens, Fungal Language: English Journal: Indian J Med Microbiol Journal subject: Microbiology Year: 2005 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Rhinosporidiosis / Rhinosporidium / Spores, Fungal / Staining and Labeling / Humans / Antibodies, Fungal / Antigens, Fungal Language: English Journal: Indian J Med Microbiol Journal subject: Microbiology Year: 2005 Type: Article