Comparative evaluation of enzyme immunoassays based on synthetic glycoconjugates and phenolic glycolipid-I for immunodiagnosis of leprosy.
Indian J Lepr
;
1990 Jan-Mar; 62(1): 60-5
Article
in English
| IMSEAR
| ID: sea-54277
ABSTRACT
Enzyme immunoassays (EIAs) based on synthetic glycoconjugates containing the terminal monosaccharide (M-BGG) or disaccharide (ND-BSA) residue of the trisaccharide component of phenolic glycolipid-I (PGL-I), for immunodiagnosis of leprosy are described. The results of the assays were compared with that of the EIA using PGL-I. All the three assays were highly specific for leprosy. The per cent positivity of active lepromatous leprosy (LL) patients with M-BGG was 78.05 in comparison to 85.36 with ND-BSA and 82.11 with PGL-I. Similarly, the positivity of tuberculoid (TT) leprosy patients in M-BGG assay was lower than that in EIAs using ND-BSA or PGL-I. However, the difference in the positivity of individual category of leprosy patients in the three EIAs was not statistically significant. The correlation between absorbance values of leprosy sera in EIAs based on M-BGG and PGL-I, as well as that in assays using ND-BSA and PGL-I was statistically significant.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
Immunoglobulin M
/
Glycoconjugates
/
Glycolipids
/
Immunoenzyme Techniques
/
Leprosy
/
Mycobacterium leprae
/
Antigens, Bacterial
Language:
English
Journal:
Indian J Lepr
Journal subject:
Tropical Medicine
Year:
1990
Type:
Article
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