Dapsone resistance does not appear to be associated with a mutation in the dihydropteroate synthase-2 gene of Mycobacterium leprae.
Indian J Lepr
;
1999 Jan-Mar; 71(1): 11-8
Article
in English
| IMSEAR
| ID: sea-54289
ABSTRACT
Evidence suggests that resistance to dapsone (DDS) in Mycobacterium leprae is related to the enzyme dihydropteroate synthase (DHPS). Two M. leprae genes (folP-1 and folP-2) encoding DHPS-1 and DHPS-2, respectively, have been identified through the M. leprae genome project. We have studied DDS-susceptible and resistant strains of M. leprae to determine whether the DDS-resistant phenotype is associated with a mutation(s) in folP-2 and to establish the number of genomic copies of the gene encoding DHPS-2 (folP-2). RFLP analysis of genomic DNA from DDS-susceptible and resistant strains of M. leprae exhibited a unique 4.2 kb restriction fragment consistent with a single genomic copy of folP-2 in both phenotypes. DNA encoding folP-2 was amplified by PCR and sequenced from two susceptible and two resistant strains of M. leprae. The folP-2 sequences from these strains were identical indicating that resistance to DDS was not associated with mutation(s) in the gene encoding DHPS-2.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Humans
/
DNA, Bacterial
/
Drug Resistance, Microbial
/
Molecular Sequence Data
/
Amino Acid Sequence
/
Dapsone
/
Dihydropteroate Synthase
/
Leprostatic Agents
/
Animals
/
Mice
Language:
English
Journal:
Indian J Lepr
Journal subject:
Tropical Medicine
Year:
1999
Type:
Article
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