Application of filter paper method for collection of blood for MLPA test.

ABSTRACT

The results of MLPA test using serum and filter paper eluate have been compared in this paper. Testing 64 patient samples at 132 dilution, 31 were negative by both serum and eluate, 20 were positive by both, six were positive only by serum and one was positive only eluate. In six other cases eluate gave equivocal results while serum result was clearly positive. Some eluates negative at 132 dilution gave weak positive agglutination at 116 dilution.