Isolation, purification and partial chemical characterization of a lethal factor from common indian toad (Bufo melanostictus, Schneider) skin extract.
Indian J Exp Biol
;
2001 Aug; 39(8): 781-5
Article
in English
| IMSEAR
| ID: sea-55691
ABSTRACT
Indian toad (Bufo melanostictus, Schneider) skin extract (TSE) is pharmacologically potent and probably contains several bioactive compounds [Das et. al., Indian J Pharmacol, 28 (1996) 72]. A lethal factor was isolated and purified by neutral alumina column chromatography followed by HPLC. Spectroscopic (UV, IR, FAB-MASS) study indicated that the lethal factor (TSE-LF) was a 254 Da long chain compound with carbonyl, hydroxyl and ester as functional groups. LD50 of TSE-LF was found to be 3.5 mg/kg (iv). Biological study showed that TSE-LF possesses hypotensive, cardiotoxic, neurotoxic activity and produced death by apnoea in experimental animal. Cyproheptadine antagonised TSE-LF induced contraction of isolated smooth muscle indicating involvement of histamine/serotonin receptors. TSE-LF induced neurotoxic action on chick biventer cervices was mediated through Ca2+ ion.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rats
/
Skin
/
Spectrum Analysis
/
Bacterial Toxins
/
Bufonidae
/
Cardiovascular System
/
Carrier Proteins
/
Chromatography, High Pressure Liquid
/
Guinea Pigs
/
Lethal Dose 50
Language:
English
Journal:
Indian J Exp Biol
Year:
2001
Type:
Article
Similar
MEDLINE
...
LILACS
LIS