Investigations on wound healing by using amphibian skin.

ABSTRACT

The present investigation is aimed to justify the validity of traditional practice of using the frog skin in wound healing. Studies were carried out on skin wounds from female albino rats of Wistar strain weighing 100-120 g. The wounds of standard size (5 cm2) were created in the experimental and control groups. The experimental group was dressed with dorsal skin of freshly sacrificed frogs (Rana tigrina) while the control group was dressed with cotton gauze. A faster healing was observed in the experimental group over the control group. Biochemical estimations of the wound granulation tissue were carried out every 2 days till the complete healing of the wound in both the groups. The collagen of frog skin was extracted and polyacrylamide gel electrophoresis was carried out to characterize and identity the molecular organization of collagen type.