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Isolation and characterization of a green tissue-specific promoter from pigeonpea [Cajanus cajan (L.) Millsp.].
Indian J Exp Biol ; 2005 Apr; 43(4): 369-72
Article in English | IMSEAR | ID: sea-56216
ABSTRACT
Expression of rbcS genes encoding small subunit of rubisco, most abundant protein in green tissue, is regulated by at least three parameters--tissue type, light conditions and stage of development. One of the green tissue-specific promoters of rbcS gene family was isolated from pigeonpea by PCR. Expression of uidA gene encoding beta-glucuronidase in the transgenic tobacco plants under the control of pigeonpea rbcS promoter, clearly showed that this promoter was as strong as pea rbcS3A promoter characterized earlier. Study of the sequence similarity with pea rbcS3A promoter, especially the region (boxes I and III) that is required for rbcS3A expression, showed more than 50% divergence. In contrast, pigeonpea promoter sequence isolated in the present study was more similar to that of spinach and rice rbcS promoters.
Subject(s)
Full text: Available Index: IMSEAR (South-East Asia) Main subject: Molecular Sequence Data / Base Sequence / Promoter Regions, Genetic / Plants, Genetically Modified / Genes, Plant / Plant Leaves / DNA, Plant / Cajanus Language: English Journal: Indian J Exp Biol Year: 2005 Type: Article

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Full text: Available Index: IMSEAR (South-East Asia) Main subject: Molecular Sequence Data / Base Sequence / Promoter Regions, Genetic / Plants, Genetically Modified / Genes, Plant / Plant Leaves / DNA, Plant / Cajanus Language: English Journal: Indian J Exp Biol Year: 2005 Type: Article