Primary cell culture from fish gills and kidney using fish serum.
Indian J Exp Biol
;
2001 Sep; 39(9): 936-8
Article
in English
| IMSEAR
| ID: sea-56234
ABSTRACT
A novel, cost effective and time saving technique for primary cell culture from gills and kidney using fish serum has been developed. Single cell suspension of gills and kidney of Clarias gariepinus was obtained by trypsinization. Minimum essential medium supplemented with 10% fish serum in place of 10% foetal calf serum and 20% fish muscle extract, yielded confluent monolayer on 6th and 8th day in gill and kidney tissue respectively at 28 degrees C. Fish serum can be successfully used as media supplement for cultivation and maintenance of primary cell culture from fishes.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Cell Culture Techniques
/
Culture Media
/
Fishes
/
Gills
/
Kidney
/
Animals
Language:
English
Journal:
Indian J Exp Biol
Year:
2001
Type:
Article
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