Cholesterol side-chain cleavage by immobilized cells of Rhodococcus equi DSM 89-133.
Indian J Exp Biol
;
1993 Apr; 31(4): 319-22
Article
in English
| IMSEAR
| ID: sea-56643
ABSTRACT
Side-chain cleavage of sterol and extracellular cholesterol oxidase activity were investigated using viable cells of R. equi DSM 89-133 immobilized in polyacrylamide gel. In batch culture, immobilized cells were active in side-chain cleavage of cholesterol for more than 30 days. Free or immobilized cells were incapable of side-chain cleavage in the absence of 2,2' dipyridyl; cholesterol oxidase was, however, produced in both the cases. Maximal activity of the immobilized cells was 60 to 70% of the free cells.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Temperature
/
2,2'-Dipyridyl
/
Cholesterol
/
Cholesterol Oxidase
/
Rhodococcus equi
/
Dose-Response Relationship, Drug
/
Fermentation
Language:
English
Journal:
Indian J Exp Biol
Year:
1993
Type:
Article
Similar
MEDLINE
...
LILACS
LIS