Isolation, purification and immunological evaluation of toxin Hb from scorpion Heterometrus bengalensis (C.L. Koch) venom.
Indian J Exp Biol
;
1990 Feb; 28(2): 144-8
Article
in English
| IMSEAR
| ID: sea-56787
ABSTRACT
Toxin-Hb, a lethal toxic antigenic protein, isolated from the venom of H. bengalensis by CM-cellulose ion-exchange chromatography was a heat labile basic protein with a molecular weight of 10 kDa. It produced irreversible blockade on the isolated rat phrenic nerve diaphragm and chick biventer cervicis. LD50 of toxin Hb was 0.48 mg/kg (iv) in mice. Antiserum was raised in mice by hyperimmunization against toxin Hb. Antitoxin Hb antiserum was immunologically potent as revealed by immunogel-diffusion and immunoelectrophoresis. Five fold protection against the lethal action of toxin Hb was achieved by the antiserum. It also effectively antagonised toxin Hb induced neuromuscular blockade on isolated rat phrenic nerve diaphragm and chick biventer cervicis preparations.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Rats
/
Scorpion Venoms
/
Scorpions
/
Temperature
/
Chickens
/
Chromatography, Ion Exchange
/
Immune Sera
/
Animals
/
Mice
Language:
English
Journal:
Indian J Exp Biol
Year:
1990
Type:
Article
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