Isolation of poly (A)+ mRNA for downstream reactions from some medicinal and aromatic plants.
Indian J Exp Biol
;
2005 Feb; 43(2): 197-201
Article
in English
| IMSEAR
| ID: sea-56941
ABSTRACT
In the present protocol for extraction of RNA, hexadecyltrimethylammoniumbromide (CTAB) and insoluble polyvinylpyrrolidone were used followed by LiCl precipitation, CsCl ultracentrifugation and finally poly (A)+ mRNA was isolated with the help of oligo(dT)-cellulose columns. The isolated poly (A)+ mRNA was found to be suitable for cDNA-AFLP and suppression subtractive hybridization applications. It is a modified and consolidated protocol based on previously described methods for isolated steps and works better for medicinal and aromatic plants. High yield of poly (A)+ mRNA coupled with its amenability for downstream reactions like RT-PCR, northern blotting and cDNA synthesis for library construction is a key feature of the present protocol.
Full text:
Available
Index:
IMSEAR (South-East Asia)
Main subject:
Plants, Medicinal
/
Reference Standards
/
RNA, Messenger
/
Plant Extracts
/
RNA, Plant
/
Catharanthus
Language:
English
Journal:
Indian J Exp Biol
Year:
2005
Type:
Article
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